Fecha/hora: 03/06/2024 14:38:52

Biblioteca(s): | INIA Treinta y Tres.
Tipo de la producción científica: | Artículos en Revistas Indexadas Internacionales
Circulación/Nivel: | -- - --
Fecha corriente: | 08/01/2021
Fecha de la última actualización: | 08/01/2021
Autoria: | RIVAS, F.; HAMPTON, J.G.; ALTIER, N.; SWAMINATHAN, J.; ROSTÁS, M.; WESSMAN, P.; SAVILLE, D. J.; JACKSON, T. A.; JACKSON, M. A.; GLARE, T. R.
Afiliación: | FEDERICO RIVAS FRANCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay // Bio-Protection Research Center, Lincoln University, Lincoln, New Zealand.; JOHN G. HAMPTON, Bio-Protection Research Center, Lincoln University, New Zealand.; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JAYANTHI SWAMINATHAN, Faculty of Agriculture and Life Sciences, Lincoln University, New Zealand.; MICHAEL ROSTÁS, Bio-Protection Research Center, Lincoln University, New Zealand.; PER WESSMAN, Department Chemical Process and Pharmaceutical Development, Unit Formulation, Research Institute os Sweden (RISE), Sweden.; DAVID J. SAVILLE, Savile Statistical Consulting Limited, Lincoln, New Zealand.; TREVOR A. JACKSON, AgResearch, Lincoln Research Center, Lincoln, New Zealand.; MARK A. JACKSON, United State Department of Agriculture (USDA), National Center for Agricultural Utilization, Peoria, IL, USA.; TRAVIS R. GLARE, Bio-Protection Research Center, Lincoln University, Lincoln, New Zealand.
Título: | Production of Microsclerotia from entomopathogenic fungi and use in maize seed coating as delivery for biocontrol against Fusarim graminearum.
Año de publicación: | 2020
Fuente/Imprenta: | Frontiers in Sustainable Food Systems, December, 2020, volume 4, Article number 606828, Pages 1-13.Open Access. Doi: https://doi.org/10.3389/fsufs.2020.606828
DOI: | 10.3389/fsufs.2020.606828
Idioma: | Inglés

Notas:

Article history: Received: 15 September 2020 / Accepted: 30 October 2020 / Published: 10 December 2020.
Correspondence: Federico Rivas-Franco frivas@inia.org.uy

Contenido: | The commercial use of the entomopathogenic fungi Metarhizium spp. in biopesticides has gained more interest since the discovery that several species of this genus are able to colonize roots. In general, commercial products with Metarhizium are formulated based on conidia for insect pest control. The process of mass production, harvesting, and formulation of infective conidia can be detrimental for conidial viability. Entomopathogenic fungi such as Metarhizium spp. are able to produce high concentrations of resistant structures, known as microsclerotia, when grown in liquid media. Microsclerotia are desiccation tolerant, with excellent storage stability, and are capable of producing high quantities of infective conidia after rehydration. The aim of this study was to evaluate microsclerotia production by different isolates of Metarhizium spp. and determine the effect of microsclerotia coated onto maize seeds on plant growth in the presence of soil-borne pathogen Fusarium graminearum. On average, ~1 × 105 microsclerotia/mL were produced by selected isolates of M. anisopliae (A1080 and F672) and Metarhizium robertsii (F447). Microsclerotia were formulated as granules with diatomaceous earth and used for seed coating, after which propagules produced around 5 × 106 CFU/g of seeds. In the presence of the plant pathogen, maize plants grown from untreated seeds had the lowest growth, while plants treated with the Metarhizium microsclerotia had significantly greater growth than the control plants. Hyphae were observed growing on and in root tissues in all the Metarhizium spp. treatments but not in samples from control plants. Metarhizium hyphal penetration points' on roots were observed 1 month after sowing, indicating the fungi were colonizing roots as endophytes. The results obtained indicate that microsclerotia can be coated onto seeds, providing plant protection against soil plant pathogens and a method to establish Metarhizium in the ecto- and endo-rhizosphere of maize roots, allowing the persistence of this biocontrol agent.
Palabras-Clave: | BIOCONTROL; ENDOPHYTIC ENTOMOPATHOGENIC FUNGI; FUSARIUM GRAMINEARUM; METARHIZIUM SPP; MICROSCLEROTIA; PLANT GROWTH PROMOTION; PLANT PROTECTION; SEED COATING.
Categoría del tema: | H20 Enfermedades de las plantas

URL:

http://www.ainfo.inia.uy/digital/bitstream/item/14918/1/fsufs-04-606828-Rivas.pdf
https://www.frontiersin.org/articles/10.3389/fsufs.2020.606828/full

Registro original: | INIA Treinta y Tres (TT)
Ejemplares: | TT - (103210-1) - PXI - AP - DD - PP/FSFS/2020/Rivas -  -  - DIS